HPV

Indication

Suspicious cytology, condyloma acuminate

Benefit

FML now offers HPV genotyping twice weekly. This test has improved sensitivity and specificity and provides a more accurate tool for diagnosis of HPV than direct hybridization methods. One sample can be used for cytology and HPV genotyping.

Background

There are various types of the human Papillomavirus and most types are transmitted sexually. Many of these do not show symptoms, do not cause disease and disappear after 1-2 years. Of the types of HPV, some carry a high oncogenic risk, others a low oncogenic risk. Some of the high-risk HPV types (HR-HPV) may progress to pre-cancerous lesions and invasive cancer.

HR-HPV are the cause of nearly all cases of cervical cancer and cervical intraepithelial neoplasia.

Material

Genital swab, brush or liquid cytology sample

Method

PCR and hybridization 

Results

The following relevant HPV types are detectable:

High oncogenic risk

16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, 82, 85

Low oncogenic risk

6, 11, 40, 42, 43, 44, 54, 61, 62, 71, 72, 81, 83, 84, 89

Preanalytics

Standard dry swab (cotton) or dried brush (from conventional Pap smears) in a dry transportation tube for PCR examination (without additional substances or liquids), do not freeze. Liquid cytology solution (Thinprep, Surepath etc.) can be used simultaneously for cytology and HPV genotyping (one vial)!

Sample Reports

Download HPV Genotyping report